Totalseq hashtagの配列

Totalseq A	Read 2 GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT (PCR handle), and BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A (capture sequence).
Totalseq B	Read2 GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (barcode) NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence)
Totalseq C	CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (barcode）NNNNNNNNNCCCATATAAGA*A*A

10x Gel beadにくっついているBarcode

RNA用

Truseq read 1

CTACACGACGCTCTTCCGATCT-N16-N12-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’

Feature barcode用

Nextera read 1

GTCAGATGTGTATAAGAGACAG-N16-N12-TTGCTAGGACCGGCCTTAAAGC-3

Template switching oligo

-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3