Quantitation of Apoptosis and Necrosis by Annexin V Binding, Propidium Iodide Uptake, and Flow Cytometry

Quantitation of Apoptosis and Necrosis by Annexin V Binding, Propidium Iodide Uptake, and Flow Cytometry

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正常な細胞は細胞膜の内部と外部がAssymmetry

PSは細胞膜の内側にしかない。

apoptosisが起こると、外部に出てくる。Annexin VはPSにくっつく

PI = only necrotic cell

Annexin V = apoptosis + necrosis