Fixed single-cell RNA sequencing for understanding virus infection and host response

Fixed single-cell RNA sequencing for understanding virus

infection and host response

https://www.biorxiv.org/content/10.1101/2020.09.17.302232v1.full.pdf

1．FD-seq, fixed single cell sequencing

2. FD-seqは,Crosslinkを破るステップをドロップレット内で行う。

3．４％ＰＦＡでＦｉｘ、Wash buffer (PBS with 1% BSA and RNase inhibitor, murine)で洗浄、Ｔｒｉｔｏｎでpermeabilization,

Proteinase Kが重要