Alignment start position on the query sequence クェリのスタートポジション
Alignment stop position on the query sequence クエリのエンドポジション
Alignment start position on the reference sequence リファレンスのスタートポジション
Alignment stop position on the reference sequence リファレンスのエンドポジション
Not used 使われない
Not used 使われない
Alignment score アライメントスコア
Query strand クエリの向き
Reference sequence strand リファレンスの向き
Query/read length クエリの長さ
Alignment as extended BTOP string This is the same BTOP string as in BLAST tabular output with a few extensions: ？
a number represents this many matches,
two bases represent a mismatch and show query and reference base,
base and gap or gap and base, show a gap in query or reference,
^<number>^ represents an intron of this number of bases,
_<number>_ represents an insertion (gap in reference) of this number of bases,
%<number>% represents a deletion (gap in read) of this number of bases,
(<number>) shows number of query bases that are shared between two parts of a spliced alignment; used when proper splice sites were not found
Number of different alignments reported for this query sequence
Not used
Compartment - a unique identifier for all alignments that belong to a single fragment. These can be two alignments for a pair of reads or alignments to exons that were not spliced.
Reverse complemented unaligned query sequence from the beginning of the query, or ‘-‘ if the query aligns to the left edge
Unaligned sequence at the end of the query, or ‘-‘
Reference sequence identifier where the mate is aligned, if different from the identifier in column 2, otherwise ‘-‘
Alignment start position on the reference sequence for the mate, or ‘-‘ if no alignment for the mate was found; a negative number denotes a divergent pair
Composite alignment score for all exons that belong to the fragment